MENG Qingyong,CHEN Shali,LIU Shuzheng.Expression of proteins in mouse splenic cells after low dose radiation and their biological activity[J].Chinese Journal of Radiological Medicine and Protection,2000,20(4):228-231 |
Expression of proteins in mouse splenic cells after low dose radiation and their biological activity |
Received:July 24, 1999 |
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KeyWords:Low dose radiation Protein expression High performance liquid chromatography |
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Abstract:: |
Objective In order to investigate the mechanisms of the hormetic effect of low dose ionizing radiation, the induction of protein expression in mouse splenic cells after whole-body irradiation was studied. Methods Expression of proteins and their biological activity was detected using gel filtration and high performance liquid chromatography (HPLC) as well as mouse splenic lymphocyte transformation with 3H-TdR incorporation and chromosome aberrations of human lymphocytes were observed. Results The changes in expression of three polypeptides with MW 18500, 17000 and 50000 were respectively detected in the crude extracts of the whole cell, cytoplasm and nucleus using HPLC.The 17 kDand 18.5 kDmolecules were down-regulated, while the 50 kDmolecule was up-regulated by low dose X-irradiation.The proliferative response of splenic lymphocytes from normal mice to ConAwas significantly enhanced after the addition of protein fractions containing the reduced amount of 17 kDand 18.5 kDmolecules, but not after the addition of the fraction containing increased amount of 50 kDmolecules.The fractions with down-regulated expression of 18.5 kDprotein and with up-regulated expression of 50 kDprotein showed a protective effect on the chromosome damage caused by radiation. Conclusion The changes in expression of protein molecules might play an important role in the mechanism of radiation hormesis. |
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