| LIU Shengxue,CAO Jia,AN Hui,et al.Molecular analysis of 60Co gamma-ray-induced mutation at HPRT locus in human promyelocytic leukemia cells[J].Chinese Journal of Radiological Medicine and Protection,2000,20(4):225-227 |
| Molecular analysis of 60Co gamma-ray-induced mutation at HPRT locus in human promyelocytic leukemia cells |
| Received:August 02, 1999 |
| DOI： |
| KeyWords:HPRT gene 60Co γ-rays Mutagenesis Multiplex PCR |
| FundProject:国家自然科学基金资助项目(39970650) |
| Author Name | Affiliation | | LIU Shengxue | Department of Molecular Toxicology, Third Military Medical University, Chongqing 400038, China | | CAO Jia | Department of Molecular Toxicology, Third Military Medical University, Chongqing 400038, China | | AN Hui | Department of Molecular Toxicology, Third Military Medical University, Chongqing 400038, China | | 周紫恒 | Department of Molecular Toxicology, Third Military Medical University, Chongqing 400038, China | | 孙华明 | Department of Molecular Toxicology, Third Military Medical University, Chongqing 400038, China |
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| Abstract:: |
| Objective To explore the spectra and mechanism of human HPRTgene mutation induced by 60Co γ-rays and its relation with antitumor effect of radiation.Methods Single cell clone culturing, twoway screening count, multiplex PCRamplification and electrophoresis technique were used. Results (1)When doses were increasing, cell plating efficiency reduced and mutation frequency increased.(2)The most frequent spontaneous mutations were point mutation (92.3%) and gammarayinduced mutation, including mainly partial deletion and point mutation(61.7% and 38.3%, respectively).(3)There were deletion mutations in all 9 exons of HPRTgene and the most of gammarayinduced mutations were chain deletion with multiplex exons (97.9%).Conclusion The spectra of spontaneous and gammarayinduced mutants were different.The bigger changes in genetic structure are related to the antitumor mechanism of radiation. |
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