| LI Yu,ZHAO Fang,ZHENG Xiulong,et al.Wortmannin inhibits repair of DNA double strand breaks induced by radiation.[J].Chinese Journal of Radiological Medicine and Protection,2000,20(3):176-178 |
| Wortmannin inhibits repair of DNA double strand breaks induced by radiation. |
| Received:December 08, 1999 |
| DOI： |
| KeyWords:Wortmannin Radiosensitization DNA double strand break |
| FundProject: |
| Author Name | Affiliation | | LI Yu | Department of Radiation Medicine, The Second Military Medical University, Shanghai 200433, China | | ZHAO Fang | Department of Radiation Medicine, The Second Military Medical University, Shanghai 200433, China | | ZHENG Xiulong | Department of Radiation Medicine, The Second Military Medical University, Shanghai 200433, China | | 罗成基 | 第三军医大学防原教研室 | | 蔡建明 | 200433 上海, 第二军医大学放射医学教研室 |
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| Abstract:: |
| Objective To study the effect of the specific inhibitor Wortmannin(WT)on repair of radiation damage of thymocyte DNA in mice. Method The cells were exposed to WT or DMSO(solvent)for 1 h, and then irradiated at the indicated doses.After indicated times post irradiation, the cells were washed to remove the WT, their DNA was made out, then DNA damage and repair were detected by electrophoresis. Results After 10 Gy and 20 Gy γ irradiation, dsb level was higher in WT group than in control group.It was more evident at 1-3 hrs after irradiation. Conclusion The radiosensitizing effect of WT is related to its inhibition of dsb repair after irradiation. |
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