| MAO Jianping,DONG Yan,LIU Bin.Development of a human somatic mutation detection method-GPA assay[J].Chinese Journal of Radiological Medicine and Protection,2000,20(3):164-167 |
| Development of a human somatic mutation detection method-GPA assay |
| Received:February 10, 1999 |
| DOI： |
| KeyWords:Glycophorin A Erythrocytes Mutation Assay |
| FundProject:解放军总后“九·五”课题基金资助项目(9605002) |
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| Abstract:: |
| Objective To study the damage to human body caused by environmental radiation, and supervise the somatic mutations. Methods Three monoclonal antibodies specific to Mtype(3G4), Ntype(6A8), and MNtype (3C5) of glycophorin A, respectively, were prepared.Fluorescence or biotin conjugated antibodies were bound specifically to formalin and/or dimethyl suberimidate fixed erythrocytes.M, MN, and N type cells were divided by cytometry to demonstrate the erythrocyte mutation characteristics (MN→MO, MM, NO, NN) and give out the variant frequency. Results 1Wa, 1Wb and 2Wa methods of GPA assay were developed.Erythrocytes of MN type individuals could be separated to normal and single locus variant groups by 1W methods and they could be sorted as normal (MN), single gene deletion mutants (MO, NO), homozygous mutants (MM, NN) cell groups by 2Wa method. Conclusion The assay is applicable to evaluationg the frequency of variant erythrocytes from human somatic mutation. |
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