| CAO Jianping,Hideyuki MAJIMA,Chizuru YAMAGUCHI.PFGE analysis of DNA double-strand breaks and DNA repair process in human osteosarcoma cells irra-diated by X-rays[J].Chinese Journal of Radiological Medicine and Protection,2000,20(3):159-163 |
| PFGE analysis of DNA double-strand breaks and DNA repair process in human osteosarcoma cells irra-diated by X-rays |
| Received:February 17, 2000 |
| DOI： |
| KeyWords:Osteosarcoma Xray irradiation Pulsedfield gel electrophoresis DNA doublestrand breaks DNA repair |
| FundProject:日本科技厅研究基金资助项目(497009) |
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| Abstract:: |
| Objective To study the induction of DNA doublestrand breaks (DSBs) in human osteosarcoma cells irradiated by Xray, the DNA DSBs repair process and the tumour cell radiosensitivity. Methods Two cell lines of human osteosarcoma, Rho0 and 14.3B were used.Initial DNA damage of DSBs by X-ray irradiation was measured using clamped homogeneous electrical field (CHEF) electrophoresis. Results X-rayinduced DNA DSBs of human osteosarcoma cells after CHEFelectrophoresis increased linearly with the irradiation dose between 0 and 50 Gy.The repair of DNA DSBs in human osteosarcoma cells increased with the postirradiation incubation time.In contrast to 14.3B cell line at the same dose point, much more DNA DSBs were induced in Rho0 cell line after X-ray irradiation. Conclusion CHEF pulsedfield gel electrophoresis (PEGE) is a sensitive method for the determination of radiationinduced DNA DSBs in high molecular weight DNA of human osteosarcoma cells.Radiationinduced DNA DSBs of osteosarcoma increase with the dose in a linear manner.after incubation, both Rho0 cell line and 143.B cell line can repair the DNA DSBs.Between two cell lines of human osteosarcoma, Rho0 and 14.3B, Rho0 cell line is more sensitive to ionizing radiation than 14.3B line. |
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