| MENG Xiangbing,SONG Yi,MAO Jianping.Participation of ATM in cellular response to DNA damage induced by ionizing radiation[J].Chinese Journal of Radiological Medicine and Protection,2000,20(3):149-152 |
| Participation of ATM in cellular response to DNA damage induced by ionizing radiation |
| Received:December 08, 1999 |
| DOI： |
| KeyWords:ATM P13K Domain LDPCR Yeast twohybrid system |
| FundProject:国家自然科学基金资助项目(39700038)全军医药卫生九五重点基金资助项目(9605007) |
| Author Name | Affiliation | | MENG Xiangbing | Department of Biochemistry, Institute of Radiation Medicine, Beijing 100850, China | | SONG Yi | Department of Biochemistry, Institute of Radiation Medicine, Beijing 100850, China | | MAO Jianping | Department of Biochemistry, Institute of Radiation Medicine, Beijing 100850, China | | 巩波 | 兰州大学生物系 | | 董燕 | Department of Biochemistry, Institute of Radiation Medicine, Beijing 100850, China | | 刘斌 | Department of Biochemistry, Institute of Radiation Medicine, Beijing 100850, China | | 孙志贤 | Department of Biochemistry, Institute of Radiation Medicine, Beijing 100850, China |
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| Abstract:: |
| Objective To clone ATM full length cDNA and cDNA fragments containing some functional domains and to identify proteins that interact with ATM and mediate DNA damage signal transduction in cellular response to DNA damage. Methods ATM cDNA was amplified from Marthom TM Ready cDNA kit of human leukocytes by LDPCR.ATMinteracting proteins were screened by yeast two hybrid system. Results ATM fulllength cDNA and cDNA fragments containing PI3K kinase domain, leucine zipper and proline rich region were amplified from human cDNAs.Several candidate clones that interacted with ATM PI3K domain were identified. Conclusion ATM mediates DNA damage signal transduction by interacting with many proteins. |
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