| PENG Ruiyun,WANG Dewen,XIONG Chengqi.In situ PCR detection and significance of IL-3 gene expression in irradiated hematopoietic cells of mouse bone marrow[J].Chinese Journal of Radiological Medicine and Protection,2000,20(2):91-93 |
| In situ PCR detection and significance of IL-3 gene expression in irradiated hematopoietic cells of mouse bone marrow |
| Received:July 21, 1998 |
| DOI： |
| KeyWords:Radiation Bone marrow Interlenkin-3 In situ PCR |
| FundProject: |
| Author Name | Affiliation | | PENG Ruiyun | Institute of Radiation Medicine, Beijing 100850, China | | WANG Dewen | Institute of Radiation Medicine, Beijing 100850, China | | XIONG Chengqi | Institute of Radiation Medicine, Beijing 100850, China | | 高亚兵 | Institute of Radiation Medicine, Beijing 100850, China | | 李延平 | Institute of Radiation Medicine, Beijing 100850, China | | 杨红 | Institute of Radiation Medicine, Beijing 100850, China | | 崔玉芳 | Institute of Radiation Medicine, Beijing 100850, China |
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| Abstract:: |
| Objective To study the significance of endogenous interleukin 3(IL-3) gene expression in repair of irradiated mouse bone marrow. Methods Seventy-eight LACA mice were subjected to total body irradiation with 60 Co γ-rays and were sacrificed within 4 weeks after irradiation.The bone marrow histopathological sections were stained with HE, and the expression of endogenous IL-3 gene was detected by means of immunocytochemistry, in situ hybridization(ISH) and in situ reverse transcription PCR(IS RT-PCR). Results Obvious injury of bone marrow occurred after irradiation and then recovered within 4 weeks.IL-3 protein was obviously increased in the cytoplasma of recovering hematopoietic cells(HCs),especially on day 21 after irradiation,while its mRNA was poorly positive by ISH on days 10-21,especially day 15.IS RT-PCR showed that IL-3 mRNA was strongly positive in recovering HCs cytoplasma,especially on days 10 to 15. Conclusion In situ RT-PCR can objectively reflect the regulation of IL-3 gene expression in bone marrow after irradiation,and the expression of endogenous IL-3 gene may play an important role in hematopoietic reconstruction of irradiated bone marrow. |
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