| JIANG Guochun,YUAN Lizhen,WEI Kang,et al.Radioresistance and cell cycle distribution of HT1080R cells after γ-ray irradiation[J].Chinese Journal of Radiological Medicine and Protection,2000,20(2):80-83 |
| Radioresistance and cell cycle distribution of HT1080R cells after γ-ray irradiation |
| Received:September 10, 1999 |
| DOI： |
| KeyWords:Radiosensitivity Cell cycle p53 |
| FundProject: |
| Author Name | Affiliation | | JIANG Guochun | Institute of Radiation Medicine, Beijing 108500, China | | YUAN Lizhen | Institute of Radiation Medicine, Beijing 108500, China | | WEI Kang | Institute of Radiation Medicine, Beijing 108500, China | | 郭学敏 | Institute of Radiation Medicine, Beijing 108500, China | | 张雪峰 | Institute of Radiation Medicine, Beijing 108500, China |
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| Abstract:: |
| Objective To investigate radioresistance and cell cycle distribution of HT1080R cells after γ ray irradiation. Methods By comparison of the survival fraction between HT1080R and HT1080 using cloning assay,cell cycle distribution and gene status were investigated by FCM and PCR-SSCP,respectively. Results HT1080R was more radioresistant than its parental cell line HT1080,and after exposure to 4 Gy and 6 Gy γ-rays showed G 2 phase arrest.PCR-SSCP showed that the expression of p53 gene was normal. Conclusion G2 arrest can be found after 4 Gy and 8 Gy γ ray irradiation in HT1080R cell line,which is more radioresistant than its parental cell line HT1080,suggesting that the acquirement of radioresistance may be related to its G 2 arrest.Because of its normal gene status and expression,radioresistance of HT1080R cannot be explained by abnormal function of p53 gene. |
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