| ZHU Shoupeng,XIAO Dong.Effect of 147Pm β-irradiation on bcl-2 gene and bax gene expression in human leukemia cells[J].Chinese Journal of Radiological Medicine and Protection,2000,20(1):33-35 |
| Effect of 147Pm β-irradiation on bcl-2 gene and bax gene expression in human leukemia cells |
| Received:April 01, 1999 |
| DOI： |
| KeyWords:Pm HL-60 cells Gene expression bcl-2 gene bax gene Immunohistochemistry RNA dot blot hybridization |
| FundProject:国家自然科学基金资助项目(39470389) |
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| Abstract:: |
| Objective To study the effect of exposure to a pure β radiator 147 Pm on bcl-2 gene expression in human leukemia HL-60 cells. Methods Immunohistochemical technique was used to identify the expression of bcl-2 and bax proteins in HL-60 cells,as well as RNA dot blot hybridization to show the expression of bcl-2 mRNA in HL-60 cells. Results The expression of bcl-2 protein was as high as 88% in control human HL-60 cells. Down regulation of bcl-2 protein expression which lowered to 53% was noted after exposure to147 Pm. The expression of bax protein was low in control cells, and after irradiation it did not obviously change. An obvious down regulation of bcl-2 mRNA expression was found in irradiated HL-60 cells. With increasing time of 147 Pm exposure, the down regulation of bcl-2 became more evident. Conclusion The apoptotic action of 147 Pm β-rays in human HL 60 cells is associated with the down regulation of the apoptosis suppressing gene bcl-2. |
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