| LI Gang,WU Dechang,HU Yingchun.Cloning of genes involved in neoplastic transformation induced by high-LET radiation[J].Chinese Journal of Radiological Medicine and Protection,2000,20(1):19-24 |
| Cloning of genes involved in neoplastic transformation induced by high-LET radiation |
| Received:June 10, 1999 |
| DOI： |
| KeyWords:Alpha particles mRNA differential display Neoplastic transformation Tumor related gene |
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| Abstract:: |
| Objective To clone genes involved in neoplastic transformation induced by α particles. Methods Differential display technique was used to identify differentially exprssed RNA species between primary and neoplastic transformed rat tracheal epithelial(RTE) cells. Results Fifteen differentially displayed gene fragments were cloned and sequenced.Among them seven novel gene sequences were submitted to GenBank.Homology searching revealed that Clone ZG 35 was highly homologous to rat Annexin Ⅰ gene which has been thought to be involved in mitogenic signal transduction as a substrate of EGF receptor kinase, Clone ZC 52 was nearly identical to a novel rattus norvegicus gene for carboxylestrase precursor, and Clone ZC 66 was highly homologous to rat NDPK gene or metastasis suppressor gene nm23. Sequence analysis suggested that nm23 may be mutated in the transformed RTE cells.and Clone ZA73 was a novel gene fragment.Screening a rat cDNA library using the novel gene Clone ZG52 (Accession number:AF007838) as probe permitted the cloning of a 3.36-kb gene.Sequence analysis and homology searching indicated that the gene contained a unique 1409bp 5'sequence and a 3' sequence showing high homology toward SRPK2,suggesting that it might be a novel SRPK2related gene involved in premRNA splicing.Northern hybridization confirmed that the above gene or gene fragments were upregulated in the transformed RTE cells. Conclusion These results provide clues to elucidate the mechanisms of radiation oncogenesis and suggest that Annexin Ⅰ,nm23,and carboxylesterase precursor gene might be involved in the neoplastic transformation induced by α-partcle radiation. |
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