| ZHAO Jingyong,LAO Qinhua,XU Yongzhong.HPRT gene locus mutation and chromosomal aberration in rat peripheral blood lymphocytes induced by internal exposure to radionuclides[J].Chinese Journal of Radiological Medicine and Protection,2000,20(1):15-18 |
| HPRT gene locus mutation and chromosomal aberration in rat peripheral blood lymphocytes induced by internal exposure to radionuclides |
| Received:June 10, 1999 |
| DOI： |
| KeyWords:Radionuclides Internal exposure Rat HPRT gene mutant Chromosomal aberration Doseresponse relationship |
| FundProject: |
| Author Name | Affiliation | | ZHAO Jingyong | Department of Radiotoxicology, Suzhou Medical College, Suzhou 215006, China | | LAO Qinhua | Department of Radiotoxicology, Suzhou Medical College, Suzhou 215006, China | | XU Yongzhong | Department of Radiotoxicology, Suzhou Medical College, Suzhou 215006, China | | 王六一 | Department of Radiotoxicology, Suzhou Medical College, Suzhou 215006, China |
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| Abstract:: |
| Objective HPRT gene locus mutation in rat peripheral blood lymphocytes induced by internal exposure to radionuclides was performed,and compared with chromosomal aberration. Methods Rats were injected intravenously with radionuclides of specific activity of 3.64×105 Bq/ml for the study of doseeffect relationship and 3.64×10 5,1.82×105,0.91×105,0.455×105 Bq/ml respectively for the study of dose rateeffect relationship.The blood was sampled at different times after injection.HPRT gene mutation frequency(GMF) and chromosomal aberration frequency (CAF) were examined by methods of multinucleus cell assay,cytokinesisblock micronucleus (CBMN) and chromosome aberration assay.Doseresponse functions were fitted with a computer. Results GMF and CAF did not increase with increase of dose and doserates but were clearly interrelated between GMF and CAF. Conclusion The HPRT gene mutation may be used as a biological dosimeter. |
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