| YE Fei,LIU Shuzheng.Changes in cell cycle progression of HeLaS3cells synchronized after X-ray irradiation[J].Chinese Journal of Radiological Medicine and Protection,1999,19(6):381-384 |
| Changes in cell cycle progression of HeLaS3cells synchronized after X-ray irradiation |
| Received:September 08, 1998 Revised:April 08, 1999 |
| DOI： |
| KeyWords:X-rays Cell cycle Synchronization HeLaS3 cells G2 delay |
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| Abstract:: |
| Objective To observe the effect of different doses of X-rays on cell cycle progression of synchronized HeLaS3 cells. Methods Using a double block method with thymidine and flow cytometric analysis,the changes in cell cycle progression of synchronized HeLaS3 cells were examined after 75 mGy and 2.0 Gy X irradiation in G0/G1,S and G2+M phases respectively,and the dose response relationship given in G2/M phase was analyzed after irradiation. Results The S and G2 phases occurred late 9 15h after the releasing point regardless of whether HeLaS3 cells were irradiated with 2 Gy in G0/G1,S or G2+M phases.HeLaS3 cells underwent a G2 arrest 9 and 12h after the releasing point when 75 mGy irradiation was administered in G0/G1 and G2+M phases,and this delay skipped completely at 12 and 15h,respectively.Moreover,the cell cycle progression was accelerated. However,there was no G2 delay at 9 and 11h when the cells were irradiated with 75 mGy in S phase,and in this case the cell cycle progression from G2/M to G0/G1 phase was accelerated.A study on the dose effect relationship showed that the number of G0/G1 phase cells decreased,and G2 delayed after 0.025-2 0.Gy irradiation given in G2/M phase,and the delay was dose dependent.However,the changes in the number of S phase cells were different between low (0.025-0.1 Gy) and higher(0.5-2.0 Gy) doses. Conclusion G2 delay is a very radiosensitive parameter,which occurs in HeLaS3 cells after X ray irradiation with a dose as low as 25 mGy when administered in G0/G1 and G2+M phases,but this delay only occurs with doses above 0.1 Gy when the cells are irradiated in S phase. |
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