| BAI Yunhong,WANG Dewen,CUI Xuemei.Effect of ligustrazine and diltiazem on fibroblast proliferation and expression of basic fibroblast growth factor following 60Co γ ray irradiation[J].Chinese Journal of Radiological Medicine and Protection,1999,19(6):379-380 |
| Effect of ligustrazine and diltiazem on fibroblast proliferation and expression of basic fibroblast growth factor following 60Co γ ray irradiation |
| Received:May 07, 1998 Revised:April 06, 1999 |
| DOI： |
| KeyWords:Irradiation Fibroblast Fibroblast growth factor |
| FundProject: |
| Author Name | Affiliation | | BAI Yunhong | Institute of Radiation Medicine, Beijing 100850, China | | WANG Dewen | Institute of Radiation Medicine, Beijing 100850, China | | CUI Xuemei | Institute of Radiation Medicine, Beijing 100850, China | | 宋良文 | Institute of Radiation Medicine, Beijing 100850, China | | 夏国伟 | Institute of Radiation Medicine, Beijing 100850, China | | 刘玉福 | 北京同仁医院眼科 |
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| Abstract:: |
| Objective In order to provide experimental basis for drug research of preventing and treating radiation pulmonary fibrosis,the authors observed the effect of ligustrazine and diltiazem on fibroblast proliferation and expression of basic fibroblast growth factor (bFGF) following 60 Co γ ray irradiation. Methods NIH/3T3 fibroblast proliferation and expression of bFGF were observed respectively by using MTT[3-(4,5-dimethylthiazo(-2-zyl) 2,5-diphenyl tetrazolium bromide)] method and ABC immunocytochemistry staining. Results The optical densities of bFGF were 0.71+0.22 for control group, 1.03+0.21 for irradiation group (Ⅰ), 0.47+0.15 for ligustrazine+irradiation group,1.05+0.21 for irradiation group (Ⅱ),and 2.02+0.21 for diltiazem+irradiation group.The mean optical densities of bFGF were 0.028+0.005,0.044+0.006,0.029+0.001,and 0.0036+0.003 for control group,irradiation group,ligustrazine+irradiation group,and diltiazem+irradiation group, respectvely. Conclusion Ligustrazine can inhibit the NIH/3T3 fibroblast proliferation and expression of bFGF,but diltiazem does not show such an effect. |
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