| WANG Junping,HU Chuanmin,SU Yongping.Using mRNA differential display to clone genes differentially expressed in intestinal epithelial cells induced by radiation in mice[J].Chinese Journal of Radiological Medicine and Protection,1999,19(6):372-374 |
| Using mRNA differential display to clone genes differentially expressed in intestinal epithelial cells induced by radiation in mice |
| Received:October 16, 1998 Revised:March 12, 1999 |
| DOI： |
| KeyWords:mRNA differential display Intestinal epithelial cells Radiation injury Differential expression |
| FundProject:国家自然科学基金;解放军总后勤部杰出人才基金;重庆市中青年科研基金 |
| Author Name | Affiliation | | WANG Junping | Institute of Combined Injury, The Third Military Medical University, Chongqing 400038, China | | HU Chuanmin | Institute of Combined Injury, The Third Military Medical University, Chongqing 400038, China | | SU Yongping | Institute of Combined Injury, The Third Military Medical University, Chongqing 400038, China | | 程天民 | Institute of Combined Injury, The Third Military Medical University, Chongqing 400038, China |
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| Abstract:: |
| Objective To find genes differentially expressed in intestinal epithelial cells (IECs) induced by radiation so as to elucidate the molecular mechanism of intestinal type of radiation sickness. Methods mRNA differential display was used to study the difference of expressed genes in IESs in normal and irradiated mice 3h after exposure to 12 Gy γ-rays,then differentially expressed gene was cloned and dot blot hybridization was carried out to confirm its identification. Results A differentially expressed gene induced by radiation was successfully cloned. Conclusion mRNA differential display can fully reflect the difference in expressed genes between normal and radiation injured IECs.Therefore,large-scale screening and identification of differentially expressed genes are conducive to the revealment of the molecular mechanism of IECs damage due to ionizing radiation. |
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