| XU Yongzhong,ZHAO Jingyong,LAO Qinhua.Mutations at HPRT loci and micronuclei induced by late fission products in peripheral blood lymphocytes of rats[J].Chinese Journal of Radiological Medicine and Protection,1999,19(5):337-340 |
| Mutations at HPRT loci and micronuclei induced by late fission products in peripheral blood lymphocytes of rats |
| Received:August 12, 1998 Revised:November 17, 1998 |
| DOI： |
| KeyWords:Late fission products HPRT gene mutation Micronucleus Dose effects Doserate effects |
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| Abstract:: |
| Objective The probability of mutations at HPRT loci used as a biological dosimeter was studied. Methods With the technique of multinuclear cell and cytokinesis-block micronucleus (CBMN) method, the relationships between mutations at HPRT loci, micronucleus and accmulative doses and dose-rates were studied. Results On the first day after intravenous injection of late fission products(accumulative dose 1.73cSv), by comparison with the control group, the HPRT variant frequencies, micronucleate cell frequencies and micronucleus frequencies increased significantly( P <0.01).There existed relationships between variant frequencies at HPRT locus, micronuclear cell frequencies, micronucleus frequencies and accumulative doses, which could be described by the following regression equation:Y=a+blnX, respectively.At the same accumulative dose, both the relationships between HPRT variant frequencies, micronucleus frequencies and dose-rates could be expressed by the logarithmic regression equation:Y=a+blnX.A good linear relationship between HPRT variant frequencies and micronucleus frequencies was observed. Conclusion HPRT gene locus is an endpoint very sensitive to injurious effects resulting from late fission products and has a good dose-response relationship. |
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