| FU Qiang,ZHANG Lansheng,ZHU Shoupeng.Effect of cycloheximide and actinomycin D on radionuclide 235 U induced apoptosis[J].Chinese Journal of Radiological Medicine and Protection,1999,19(5):325-327 |
| Effect of cycloheximide and actinomycin D on radionuclide 235 U induced apoptosis |
| Received:June 03, 1998 Revised:November 08, 1998 |
| DOI： |
| KeyWords:Cycloheximide Actinomycin D Radionuclide 235 U Apoptosis |
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| Abstract:: |
| Objective The mechanism of apoptosis induced by radionuclide 235 U was studied.Methods MTT and JAM assay were used to analyse the cell viability and quantification of fragmented DNA. Results The inhibitor of protein cycloheximide (CHX), and the inhibitor of RNA synthesis, actinomycin D, cannot inhibit the apoptosis induced by 235 U, but CHX can partly inhibit apoptotic cells DNA fragmentation. Conclusion The pathway of apoptosis induced by radionuclide 235 U is different from X-and γ-ray external irradiation, protein synthesis is not essential for it,but synthetic endonuclease is necessary for DNA fragmentation of apoptotic cells. |
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