| ZHANG Shanwen,XIAO Weiqun,LU Youyong.G1 arrest and apoptosis of BGC 823 cell lines with different p53 status after irradiation[J].Chinese Journal of Radiological Medicine and Protection,1999,19(5):322-324 |
| G1 arrest and apoptosis of BGC 823 cell lines with different p53 status after irradiation |
| Received:June 07, 1998 Revised:November 22, 1998 |
| DOI： |
| KeyWords:Tumor suppressor gene p53 Apoptosis Irradiation |
| FundProject:国家自然科学基金 |
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| Abstract:: |
| Objective To assess the role of the p53 tumor suppressor gene(wild-type p53) in G1 arrest and apoptosis after irradiation in human gastric carcinoma 823-cell lines(BGC823). Methods Three human gastric carcinoma 823-cell lines with different p53 status, i.e.BGC823-wtp53 cells with wild-type p53, BGC823-mutp53 cell with mutant p53 alleles and BGC823-vect without p53 gene, were used.Various cell phase distribution and apoptosis were analyzed by flow cytometry in 0, 8 and 24h following irradiation with 4Gy. Results The BGC823 wtp53 cells had strong arrest in G 1 phase at 8 and 24h following radiation with 4Gy (67 9% and 61 1% of original population, respectively), while the BGC823-mutp53 and BGC823-vect cells had almost no arrest in G 1 phase at 8 and 24h following radiation with 4Gy.In the BGC823-wtp53 cells, a sub G 1 phase peak of apoptosis could be observed at 8 and 24h after irradiation with 4Gy, apoptotic cell rates being 13 0% and 15 3%, respectively, while the BGC823-mutp53 and BGC823-vect cells had no apoptotic response after irradiation with 4Gy. Conclusion Wild type p53 genes promote G 1 phase arrest and apoptosis of tumor cells following irradiation, while those with mutant or vect p53 genes abrogate this response to irradiation. |
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