| LIANG Ke,HE Shaoqing,FENG Yan.Apoptosis of nasopharyngeal carcinoma cell line(CNE-2) induced by neutron irradiation[J].Chinese Journal of Radiological Medicine and Protection,1999,19(5):319-321 |
| Apoptosis of nasopharyngeal carcinoma cell line(CNE-2) induced by neutron irradiation |
| Received:March 21, 1999 Revised:June 13, 1999 |
| DOI： |
| KeyWords:Neutron beams Apoptosis Nasopharyngeal carcinoma |
| FundProject:国家教委博士点课题;国家自然科学基金课题;国家科委“九五”攀登计划 |
| Author Name | Affiliation | | LIANG Ke | Cancer Hospital, Chinese Academy of Medical Sciences, Beijing 100021, China | | HE Shaoqing | 上海医科大学肿瘤医院肿瘤放疗科 | | FENG Yan | 上海医科大学肿瘤医院肿瘤放疗科 | | 唐锦华 | 中国科学院高能物理研究所十一室 | | 冯勤富 | Cancer Hospital, Chinese Academy of Medical Sciences, Beijing 100021, China | | 沈瑜 | Cancer Hospital, Chinese Academy of Medical Sciences, Beijing 100021, China | | 殷蔚伯 | Cancer Hospital, Chinese Academy of Medical Sciences, Beijing 100021, China | | 徐国镇 | Cancer Hospital, Chinese Academy of Medical Sciences, Beijing 100021, China | | 刘新帆 | Cancer Hospital, Chinese Academy of Medical Sciences, Beijing 100021, China | | 王绿化 | Cancer Hospital, Chinese Academy of Medical Sciences, Beijing 100021, China | | 高黎 | Cancer Hospital, Chinese Academy of Medical Sciences, Beijing 100021, China |
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| Abstract:: |
| Objective To study the apoptotic response of the nasopharyngeal carcinoma cell line (CNE-2) induced by neutron irradiation. Methods CNE-2 cells were cultured as usual.Using the techniques of DNA agarose gel electrophoresis and DNA special fluorescent staining,the status of apoptosis in CNE-2 cells after neutron irradiation was detected. Results It was shown that the apoptosis can be induced in CNE-2 cell after neutron radiation.Six hrs, after different doses of neutron(0/0.667/1.333/2.000/ 2.667/3.333Gy) and X ray (0/2/4/6/8/10 Gy) irradiation the apoptotic rates were 2.4%, 6.3%, 7.1%, 9.5%, 13.5%, 14.6% and 2.4%, 3.8%, 5.7%, 7.8%, 10.4%, 11.7%, respectivelyat 48hrs they were 18.3%, 21.5%, 22.8%, 29.3%, 34.2% and 13.7%, 17.6%, 21.3%, 25.6%, 28.9%, respectively.At 10hrs after neutron irradiation the DNA ladder of apoptosis could be detected between 0.667- 3.333 Gy doses in CNE-2 cells by DNA agarose gel electrophoresis. Conclusion Neutron radiation can induceapoptosis in tumor cells.Compared with the X ray, neutron induces apoptosis in larger extent than X ray in the same conditionmeanwhile, apoptosis after irradiation is dose and time dependent. |
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