| HU Yingchun,ZHANG Kaitai,WU Dechang.A rapid method for screening arrayed plasmid cDNA library by PCR[J].Chinese Journal of Radiological Medicine and Protection,1999,19(5):310-312 |
| A rapid method for screening arrayed plasmid cDNA library by PCR |
| Received:October 08, 1998 Revised:February 25, 1999 |
| DOI： |
| KeyWords:Polymerase chain reaction cDNA library screening |
| FundProject:国家自然科学基金 |
| Author Name | Affiliation | | HU Yingchun | Institute of Radiation Medicine, Beijing 100850, China | | ZHANG Kaitai | Institute of Radiation Medicine, Beijing 100850, China | | WU Dechang | Institute of Radiation Medicine, Beijing 100850, China | | 李刚 | Institute of Radiation Medicine, Beijing 100850, China | | 项晓琼 | Institute of Radiation Medicine, Beijing 100850, China |
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| Abstract:: |
| Objective To develop a PCR-based method for rapid and effective screening of arrayed plasmid cDNA library. Methods The plasmid cDNA library was arrayed and screened by PCR with a particular set of primers. Results Four positive clones were obtained through about one week. Conclusion This method can be applied to screening not only normal cDNA clones, but also cDNA clones-containing small size fragments.This method offers significant advantages over traditional screening method in terms of sensitivity, specificity and efficiency. |
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