| XIANG Yingsong,YANG Rujun,CAI Jianming,et al.Scheduled transplantation of bone marrow cells preincubated with acidic fibroblast growth factor(aFGF)[J].Chinese Journal of Radiological Medicine and Protection,1999,19(5):303-306 |
| Scheduled transplantation of bone marrow cells preincubated with acidic fibroblast growth factor(aFGF) |
| Received:July 31, 1998 Revised:October 14, 1998 |
| DOI： |
| KeyWords:Bone marrow transplantation Graft versus host disease Acidic fibroblast growth factor |
| FundProject:国家自然科学基金 |
| Author Name | Affiliation | | XIANG Yingsong | Department of Radiation Medicine, Second Military Medical University, Shanghai 200433, China | | YANG Rujun | Department of Radiation Medicine, Second Military Medical University, Shanghai 200433, China | | CAI Jianming | Department of Radiation Medicine, Second Military Medical University, Shanghai 200433, China | | 李百龙 | Department of Radiation Medicine, Second Military Medical University, Shanghai 200433, China |
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| Abstract:: |
| Objective To develop a new method of bone marrow scheduled transplantation (BMST) by making use of the effects of acidic fibroblast growth factor (aFGF) on improving hematopoiesis. Methods The scheduled transplantation of bone marrow cells preincubated with aFGF (aFGF BMST) was carried out to study the effects of aFGF on hematopoietic reconstitution and reducing acute graft versus host disease (GVHD) in acute radiation disease model of Kungming mice. Results The survival rate of the group of aFGF-BMST mice with 4×106BMXs was 40%, which was higher than the survival of the group of BMT with 1×107BMCs alone (30%), but was lower than the survival of the group of BMST with 4×106 BMCs. On the other hand, the recovery rates in numbers of leucocytes, nucleated cells and CFU-E, CFU-GM, CFU-S were faster than those in the group of BMT with 1×107 BMCs alone and in the group of BMST with 4×106 BMCs.In addition, the severity of GVHD in the group of aFGF-BMST mice with 4×106 BMCs was lower than that in the group of BMT with 1×107 BMCs alone but was higher than that in the group of BMST with 4×106 BMCs. Conclusion Although aFGF can activate heterogneous T cells to cause GVHD, there is prospect of making full use of the effects of aFGF on improving hematopoisis and reducing the side effects of aFGF leading to GVHD through scheduled transplantation of bone marrow cells preincubated with aFGF. |
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