| ZHOU Ying,QU Wanying,LI Meng.Use of hypoxia imaging agent99mTc-HL91 in rat cerebral ischemia models[J].Chinese Journal of Radiological Medicine and Protection,1999,19(4):275-278 |
| Use of hypoxia imaging agent99mTc-HL91 in rat cerebral ischemia models |
| Received:April 12, 1999 Revised:May 17, 1999 |
| DOI： |
| KeyWords:Technetium HL91 Rat Cerebral ischemia |
| FundProject:国家攀登计划B项目资助 |
| Author Name | Affiliation | | ZHOU Ying | Department of Nuclear Medicine, Beijing Hospital, Beijing 100730, China | | QU Wanying | Department of Nuclear Medicine, Beijing Hospital, Beijing 100730, China | | LI Meng | 北京协和医院核医学科 | | 陈方 | 国家标准物质研究中心 | | 姚稚明 | Department of Nuclear Medicine, Beijing Hospital, Beijing 100730, China | | 朱明 | Department of Nuclear Medicine, Beijing Hospital, Beijing 100730, China | | 朱霖 | 国家标准物质研究中心 |
|
| Hits: 2493 |
| Download times: 1789 |
| Abstract:: |
| Objective To explore the possibility of diagnosis for cerebrovascular disease by a novel synthetic hypoxia agent99mTc-HL91 used in rat cerebral ischemia models. Methods Pharmacological experiments of 99mTc-HL91 were carried out including common properties, radiochemical purity, stability in vitro, anomalous toxicity test and biodistribution in mice. Fifteen cerebral ischemic rat models were established and received 99mTc-HL91 scintigraphy. Results HL91 kits were labeled with 99mTc easily and showed high radiochemical purity and stability.Rapid clearance in blood, heart and lungs and high activity in liver, kidneys and intestines were observed. Relatively low uptake in brain was identified. The radioactivity in ischemic brain tissue increased significantly at 4h postinjection in both rat images and isolated brain images.The radioactivity ratios of lesion to normal brain tissue by drawing ROIs in isolated brain planar images were 0.98±006, 0.99±0.05, 1.29±003, 1.56±0.14 and 1.66±0.06 at 1, 2, 4, 8 and 12 h postinjection, respectively. There were significant differences among all groups except for 1 h and 2 h, 8 h and 12 h postinjection (P<0.05P<0.001). The radioactivity ratios in per unit weight of ischemic to normal brain tissues were gradually increasing. Conclusion Increased retention ratio and decreased clearance rate of 99mTc-HL91 in the hypoxic, ischemic brain tissue have been proved. It is appropriate to perform imaging at 4 h postinjection. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
