| YANG Meiying,YE Changqing,YAO Shuxiang.Search for genes related to tin miners lung cancer tissues by differential display of mRNA[J].Chinese Journal of Radiological Medicine and Protection,1999,19(4):263-266 |
| Search for genes related to tin miners lung cancer tissues by differential display of mRNA |
| Received:January 10, 1999 Revised:May 04, 1999 |
| DOI： |
| KeyWords:Differential display Tin miners Lung cancer Related genes |
| FundProject:国家自然科学基金 |
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| Abstract:: |
| Objective To search for the genes related to tin miners lung cancer tissues. Methods Differential display of mRNA.] Results Thirty cDNA fragments which differentially expressed in lung cancer tissues and the same patients normal lung tissues were discovered.Among these, 16 expressed in lung cancer tissues, not in normal lung tissues;14 expressed on the contrary.Six cDNA fragment sequences were determined, of which 5 sequences coded CG2, CG7, CG8, CA5 and CC6 had less than 75% of homology with known sequences in GenBank BLAST, so they were believed to be new sequences which we have recorded in Genbank.Only one fragment sequence coded CG3 had up to 95% of homology with human ribosome protein L27a gene. Conclusion mRNA differential display provides a unique and powerful experimental system to study differential gene expression in tin miners lung cancer tissues and the same patients normal lung tissues.Using the system, differential expression of 30 cDNA fragments was observed.Six of them may be used in study on molecular mechanism of miners radonassociated lung cancer. |
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