| JU Guizhi,FU Haiqing,SU Xu.Molecular pathway of G1 arrest of thymocytes induced by ionizing radiation[J].Chinese Journal of Radiological Medicine and Protection,1999,19(4):258-260 |
| Molecular pathway of G1 arrest of thymocytes induced by ionizing radiation |
| Received:November 01, 1998 Revised:December 26, 1998 |
| DOI： |
| KeyWords:Ionizing radiation G1 arrest Pathway |
| FundProject:国家自然科学基金 |
| Author Name | Affiliation | | JU Guizhi | MH Radiobiology Research Unit, Norman Bethune University of Medical Sciences, Changchun 130021, China | | FU Haiqing | MH Radiobiology Research Unit, Norman Bethune University of Medical Sciences, Changchun 130021, China | | SU Xu | MH Radiobiology Research Unit, Norman Bethune University of Medical Sciences, Changchun 130021, China | | 傅士波 | MH Radiobiology Research Unit, Norman Bethune University of Medical Sciences, Changchun 130021, China | | 刘树铮 | MH Radiobiology Research Unit, Norman Bethune University of Medical Sciences, Changchun 130021, China |
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| Abstract:: |
| Objective To investigate the molecular pathway of G1 arrest of thymocytes induced by ionizing radiation. Methods Flow cytometry (FCM) was used to analyze cell cycle stained with propidium iodide (PI).And FCM was also used to analyze protein expression of thymocytes for their immunofluorescence stained with monoclonal antibodies. Results It was found that G1 phase cells of thymocytes increased significantly 12 h after whole body irradiation (WBI) with doses of 0.5, 1.0 and 2.0 Gy, and 24 h following 2 Gy WBI in mice.The results also showed the increases of p53+ cells of thymocytes at 1 h to 8 h, p21+ cells at 4 h to 48 h, and MDM2+ cells at 4 h and 8 h after 2 Gy WBI in mice.But no change was found in GADD45+ cells. Conclusion G1 arrest could be induced by single WBI with 0.5 Gy to 2.0 Gy X-rays, and its molecular control might be established through the p53-p21 pathway. |
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