| CAO Hua,GE Zhongliang,ZHANG Qunwei,et al.Influence of rhTPO/GMCSF fusion protein on hemopoiesis in mice irradiated with 60 Co rays[J].Chinese Journal of Radiological Medicine and Protection,1999,19(4):251-253 |
| Influence of rhTPO/GMCSF fusion protein on hemopoiesis in mice irradiated with 60 Co rays |
| Received:February 24, 1999 Revised:May 25, 1999 |
| DOI： |
| KeyWords:TPO GM-CSF rhTPO/GM-CSF Fusion protein Hematopoietic activity |
| FundProject:国家自然科学基金 |
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| Abstract:: |
| Objective To find a new biological therapy for secondary hematopoietic failure including anemia, infection and hemorrhage after administration of chemotherapeutic drugs etc. Methods hGM-CSF gene was ligated with hTPO gene isolated from human fetal liver mRNA and a new fusion protein rhTPO/GMCSF obtained. Results The new fusion protein could promote recovery of peripheral WBC and PLT of 5.0 Gy irradiated mice.BFU-E, CF-UMeg and CF-UGM in bone marrow of mice after irradiation recovered significantly by treatment with rhTPO/GM-CSF fusion protein for 10 days. Conclusion These results suggest that the new fusion protein has the biological activity of both hTPO and hGM-CSF simultaneously and can stimulate the proliferation of megakaryocyte and granulocyte progenitors. |
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