| CHEN Xinghua,LUO Chengji,GUO Chaohua.Primary observation on adherent function of bone marrow stromal cells in mice post combined radiation burn injury[J].Chinese Journal of Radiological Medicine and Protection,1999,19(3):186-190 |
| Primary observation on adherent function of bone marrow stromal cells in mice post combined radiation burn injury |
| Received:January 19, 1998 Revised:April 16, 1998 |
| DOI： |
| KeyWords:Combined radiation-burn injury Bone marrow Stromal cells Cell adherence Mice |
| FundProject:全军“八五”攻关基金 |
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| Abstract:: |
| Objective To investigate the adherent function of bone marrow stromal cells in hematopoietic inducive microenvironment post combined radiation burn injury. Methods The expression of cell adhesion molecules including vascular cell adhesion molecule 1(VCAM-1),fibronection(Fn),laminin (Ln) and collagen type IV (Col Ⅳ) on bone marrow stromal cells cultured in vitro was detected by flow cytometry and the binding capacity of bone marrow mononuclear cells to stromal cell adherence layer was tested by cell binding assay and cell binding blocking assay respectively from mice treated with 5.0 Gy γ ray 15% of total body surface area (TBSA),third degree burn injury and combined irradiation burn injury,respectively. Results 1.The expression levels of molecules mentioned above in burn injured mice were the highest.The molecules levels in control mice were greater than those in radiation injured mice,which were lower than those in mice with combined radiation burn injury.2.The binding capacity of stromal cell adherence layer in burn injured mice was greater than that in control mice,and significantly increased from 3 to 7 days post injury as compared with that in controls,radiation injured mice and combined radiation burn injured mice, respectively ( P <0.05-0.01).Contrarily,the capacity of binding in the radiation injured and combined radiation burn injured mice was the lowest from 3 to 7 days post injury.3.The binding rate of bone marrow mononuclear cells to stromal cell adherence layer descended in different degrees after pre treatment with monoclonal antibodies directed to VCAM-1,Fn,Ln,or Col Ⅳ respectively or VCAM-1 combined with anti Fn,anti Ln or anti-Col Ⅳ,respectively,in stromal cell adherence layer. Conclusion The damage of cell adherent function for bone marrow hematopoietic inducive microenvironment post combined radiation burn injury might be one of the important factors in hematopoietic disorder in combined radiation burn injury. |
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