ZHOU Xiao,WU Dechang,CHENG Shujun.Isolation and cloning of genes related to the transformation of immortalized human bronchial epithelial cell line induced by alpha particle radiation[J].Chinese Journal of Radiological Medicine and Protection,1999,19(3):177-179 |
Isolation and cloning of genes related to the transformation of immortalized human bronchial epithelial cell line induced by alpha particle radiation |
Received:February 24, 1998 Revised:July 14, 1998 |
DOI: |
KeyWords:α-Radiation Human bronchial epithelial immortalized cell line mRNA differential display Genes related to transformation |
FundProject:国家自然科学基金;中国博士后科学基金 |
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Abstract:: |
Objective To find out the genes related to transformation of human bronchial epithelial cell line and to clone them. Methods The mRNA differential display method was used. Results Differential cDNA fragments were found.After sequencing and identification in GenBank,4 fragments were found to be novel ones and were registered in EMBL database. Conclusion Four novel cDNA fragments have been found,but their biological characterictics and functions are still to be elucidated. |
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