| YUE Jingyin,LIU Bingchen,WU Hongying,et al.Genomic Instability induced by 137 Cs γ-ray irradiation in CHL surviving cells[J].Chinese Journal of Radiological Medicine and Protection,1999,19(2):112-115 |
| Genomic Instability induced by 137 Cs γ-ray irradiation in CHL surviving cells |
| Received:May 10, 1998 Revised:October 05, 1998 |
| DOI： |
| KeyWords:Genomic instability Mutationhgprt locus Micronuclei Apoptosis |
| FundProject:国家自然科学基金 |
| Author Name | Affiliation | | YUE Jingyin | Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin 300192, China | | LIU Bingchen | Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin 300192, China | | WU Hongying | Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin 300192, China | | 周继文 | Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin 300192, China | | 穆传杰 | Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin 300192, China |
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| Abstract:: |
| Objective To study in parallel several possible manifestations of instability of surviving CHL cells after irradiation,namely the frequencies of mutation at locus,micronuclei and apoptosis.Methods The frequencies of mutation at hgprt locus,micronuclei and apoptosis were assayed at various times in surviving cells irradiated with γ-rays.Results The surviving cells showed a persistently increased frequency of mutation at the hgprt locus after irradiation until 53 days.Mutant fraction as high as 10-4 was scored,tens of times higher than those assayed in control cells studied in parallel.The frequency of binucleated cells with micronuclei determined within 24 hours after irradiation increased with dose and reached a peak value of (26.58±2.48)% at 3Gy,decreasing at higher doses to a plateau around 20%.The micronucleus frequency decreased steeply to about(14.47±2.39)% within the first 3 days post irradiation,and fluctuated at around 10% up to 56 days post irradiation.The delayed efficiency of irradiated cells was significantly decreased.The frequency of apoptosis peaked about(24.90±4.72)% at 10 Gy 48 h post irradiation(γ-ray dose between 3 10 Gy)and then decreased to about 12% within 3 days.It was significantly higher than in control cells until 14 days.Conclusiones It shows that genomic instability induced by radiation can be transmitted to the progeny of surviving cells and may take many forms of expression such as lethal mutation,chromosome aberrations,gene mutation,etc. |
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