| FU Haiqing,JU Guizhi.Effect of whole body irradiation with X rays on [Ca2+]i response to Con A in splenocytes[J].Chinese Journal of Radiological Medicine and Protection,1999,19(1):27-29 |
| Effect of whole body irradiation with X rays on [Ca2+]i response to Con A in splenocytes |
| Received:September 22, 1998 Revised:December 20, 1998 |
| DOI： |
| KeyWords:Ionizing radiation Splenocytes Fura 2AM Intracellular free Ca 2+ |
| FundProject:国家自然科学基金 |
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| Abstract:: |
| Objective To elucidate the possible mechanism of immunodepression following whole body irradiation (WBI) with X rays,intracellular free Ca 2+ ([Ca2+]i) response to Con A in splenocytes of mice after WBI was studied. Method Fluorescent indicator fura-2 was used to assess the changes of [Ca2+]i response to Con A in splenocytes of Kunming mice following WBI with X-rays. Results WBI with 0,0.5,1,2,4, and 6 Gy X-rays,respectively,could inhibit dose dependently mitogen Con A-stimulated [Ca2+]i elevation and prolong the time of Con A-stimulated [Ca2+]i elevation to a peak level in splenocytes of mice 24h after irradiation.Time course of 2 Gy WBI with X-rays indicated that inhibition of Con A-stimulated [Ca 2 ] i increase in splenocytes began at 2h,reached the lowest value at 24h,and slightly recovered at 5d after WBI. Conclusion Immunodepressive effect of ionizing radiation is associated with inhibition of [Ca2+]i mobilization in T lymphocytes. |
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