| ZHU Maoxiang,GUO Renfeng,YANG Zhihua.Promotion of TNF α on malignant transformation of Syrian hamster embryo cells irradiated with α particles[J].Chinese Journal of Radiological Medicine and Protection,1999,19(1):6-10 |
| Promotion of TNF α on malignant transformation of Syrian hamster embryo cells irradiated with α particles |
| Received:June 25, 1998 Revised:August 16, 1998 |
| DOI： |
| KeyWords:Tumor necrosis factor α Syrian hamster embryo cells Alpha particle irradiation Cell transformation Protein tyrosine phosphorylation |
| FundProject:国家自然科学基金;军事医学科学院青年基金 |
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| Abstract:: |
| Objective To illustrate the role of tumor necrosis factor α(TNF-α) in radiation induced cancer and regulatory mechanism of protein tyrosine phosphorylation.Methods Taking Syrian hamster embryo cells exposed to 0.5 Gy α-particles as the target, an array of biological indicators such as cell growth curve, transformation frequency (TF), colony formation efficiency(CFE) and tumor formation in nude mice were observed, and the activities of protein tyrosine kinases and protein tyrosine phosphatases were measured.Results Neither 0.5 Gy α-particle irradiation nor TNF α alone could induce transformation of SHE cells morphologically, but the TF, CFE and levels of protein tyrosine phosphorylation were obviously increased in SHE cells treated with 600 U/ml TNF-α after exposure to 0.5Gy α-particles, and malignant transformation was proved by tumorigenicity assays.Conclusion TNF-α promotes significantly the transformation of SHE cells induced by α-particles, and protein tyrosine phosphorylation is probably involved in regulation of the process. |
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