| Li Xiaxin,Hong Ping,Cheng Shaojie,et al.Change of telomerase activity in gamma irradiated hematopoietic cells.[J].Chinese Journal of Radiological Medicine and Protection,1998,18(6):377-380 |
| Change of telomerase activity in gamma irradiated hematopoietic cells. |
| Received:April 03, 1997 Revised:November 24, 1997 |
| DOI： |
| KeyWords:Gamma rays Hematopoietic cells Telomerase Cell cycle |
| FundProject:军队“八·五”青年基金 |
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| Abstract:: |
| Objective Using two hematopoietic cell lines, KGla and CEM, to investigate the variation of telomerase activity after γ-irradiation.Methods After irradiation, cell proliferation was measured by thymidine incorporation and telomerase activity of cells was analysed by Kim's TRAP assay. Cell synchronization was achieved after two-step thymidine blocks, and cell cycle was examined by BrdU incorporation technique for verifying cell cycle distribution after irradiation and cell synchronization.Results Telomerase activity increased after irradiation of between 0 and 3Gy in a dose-dependent manner, reaching the maximum at 3 Gy. The increase of telomerase activity was nearly to its maximum 8h after irradiation, the peak being observed at around 24h. Although this kinetics partly correlated with cell redistribution into the G2/M phase of the cell cycle, telomerase activity did not show significant variation over the cell cycle.Conclusion The up-regulation of telomerase in hematopoietic cell lines after γ-irradiation may suggest the involvement of telomerase in DNA repair and chromosome healing. |
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