| Ding Guirong,Guo Yao.Effect of L-NAME on radiation induced IEC-6 cell injury[J].Chinese Journal of Radiological Medicine and Protection,1998,18(3):156-158 |
| Effect of L-NAME on radiation induced IEC-6 cell injury |
| Received:November 07, 1997 Revised:September 01, 1997 |
| DOI： |
| KeyWords:L-NAME Small intestines Epithelial cells Nitric oxide Radiation |
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| Abstract:: |
| Objective To study the effect of L-NAME on radiation induced injury of small intestinal epithelial cells 6(IEC-6).Methods Rat IEC-6 cells were irradiated with 60Coγ rays.The content of nitric oxide(NO) in media supernatant of IEC-6 cells was measured by fluorometric method.Nitric oxide synthase (NOS) activity was observed by histochemistry of NADPHdiaphase and immunocytochemistry.Cell viability was evaluated by method of MTT.Results NOgeneration by IEC-6 cells was significantly increased after irradiation.The assay of cNOSactivity showed that it was stimulated.iNOSactivity was inhibited.L-NAME was able to increase cell viability which had been reduced by irradiation, but it was reversed partly by pretreatment with NOsubstrate L Arg.Conclusion NOwas involved in IEC-6 cell injury induced by radiation and L-NAME could provide protective effect on it. |
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