| Wu Wei,Kong Xiantao,Yang Rujun.Apoptosis of thymocytes in sublethally 60 Co γ irradiated mice[J].Chinese Journal of Radiological Medicine and Protection,1998,18(2):82-85 |
| Apoptosis of thymocytes in sublethally 60 Co γ irradiated mice |
| Received:November 29, 1996 Revised:June 23, 1997 |
| DOI： |
| KeyWords:Thymocytes Apoptosis Whole body irradiation |
| FundProject:全军医药卫生科研基金 |
| Author Name | Affiliation | | Wu Wei | Clinical Immunology Center of PLA, Shanghai Changzheng Hospital, The Second Military Medial University Shanghai 200433, China | | Kong Xiantao | Clinical Immunology Center of PLA, Shanghai Changzheng Hospital, The Second Military Medial University Shanghai 200433, China | | Yang Rujun | 放射医学研究所 | | 李莉 | Clinical Immunology Center of PLA, Shanghai Changzheng Hospital, The Second Military Medial University Shanghai 200433, China | | 闵锐 | 放射医学研究所 | | 张玲珍 | Clinical Immunology Center of PLA, Shanghai Changzheng Hospital, The Second Military Medial University Shanghai 200433, China | | 施笑梅 | Clinical Immunology Center of PLA, Shanghai Changzheng Hospital, The Second Military Medial University Shanghai 200433, China | | 周炳荣 | 微生物教研室 |
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| Abstract:: |
| Objective The aim of this study was to investigate the apoptosis of thymocytes in 60 Coγ irradiated mice.Methods By flow cytometry,electrophoretic analysis of DNA and lymphocyte stimulating test,we determined the changes of thymocyte apoptosis in mice.The mice were whole body irradiated with 2.0,4.0 and 6.0 Gy 60 Coγ rays.Results While the thymocytes underwent apoptosis within 2 h after irradiation,fragmentation rate increased approximately 4～8 h after exposure to radiation doses of 2.0—6.0 Gy.Agarose gel electrophoresis of DNA in the 27 000×g supernatant of lysates obtained from thymocytes of irradiated mice showed ladder pattern.We found that PHA and rIL-2 prevented thymocytes from undergoing apoptosis induced by γ irradiation.Conclusion These observations suggest that the radiosensitivity of mouse thymocytes varied in different dose groups after irradiation.It is possible that PHA and rIL-2 exert protective effects on radiation iuduced apoptosis of thymocytes. |
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