| Li Jinying,Wei Shanjian,Hu Xiaojian.Characterization of changes in composition and function of erythrocyte membrane proteins in patients with bone marrow form of acute radiation sickness[J].Chinese Journal of Radiological Medicine and Protection,1998,18(1):39-42 |
| Characterization of changes in composition and function of erythrocyte membrane proteins in patients with bone marrow form of acute radiation sickness |
| Received:April 15, 1997 Revised:May 20, 1997 |
| DOI： |
| KeyWords:Radiation damage Erythrocyte membrane protein Anion transport |
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| Abstract:: |
| Objective The delayed effect of radiation on erythtocyte membrane protein, the composition and function of the membrane proteins in five patients with bone marrow form of acute radiation sickness(ARS) were follow up at six years after the Shanghai 60 Co irradiation accident. Methods Percoll centrifugation, SDS polyacryamide gel electrophoresis, and analysis of NO2- transport rate and DIDS inhibition rate were performed. Results The injuries of the membrane proteins induced by radiation, characterized by reduced content of band 8 and declined anion transport function of band 3 protein remained the same as initially observed.The further study showed that the inhibition of DIDS on the anion transport of the ARS erythrocytes was decreased and the transport time for NO2- by band 3 was significantly prolonged in younger erythrocytes than those in middle or old aged cells. Conclusion It is suggested that the radiation damage to erythrocyte membrane proteins might occur at the stage of erythropoiesis in bone marrow.The exofacial site in band 3 may be changed after radiation, which could result in the abnormalities in anion transport.It is believed that the aging of erythrocytes might be present in advanced stage of ARS. |
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