| Wang Bing,Keiko Watanabe,Takeshi Yamada,et al.Effects of organically bound tritium (obt) on cultured midbrain cells from embryonic mice[J].Chinese Journal of Radiological Medicine and Protection,1997,17(5):319-323 |
| Effects of organically bound tritium (obt) on cultured midbrain cells from embryonic mice |
| Received:March 04, 1996 Revised:July 09, 1997 |
| DOI： |
| KeyWords:Organically bound tritium Embryos Midbrain cells Differentiation Relative biological effectiveness Mice |
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| Abstract:: |
| Objective Four kinds of organically bound tritium compounds (OBTs) including 3H-thymidine, 3H-uridine, 3H-ariginine and 3H-glutamic acid,were investigated on proliferation and differentiation of cultured mouse embryonic midbrain cells (MBCs).Methods MBCs were isolated from day 11 embryos,cultured at a high concentration with the medium containing OBT.Results Differentiation of MBC was more sensitive to radiation than proliferation.Dose dependent decrease of DNA and protein contents were also observed.The RBE values,ranging from 4.6 to 8.7,of β rays from OBTs were obtained when compared with X irradiation at their ID50s (inhibitory dose that reduced assessment value by 50% of the control) on inhibition of cell proliferation and differentiation,and on reduction of DNA and protein contents of the cultures.The mixed exposure to X rays and one kind of OBTs or to any two kinds of OBTs resulted in more efficiently inhibitory effect on differentiation.Conclusions MBC culture system was more sensitive to beta radiation from OBTs than to X-rays,which resulted in very high RBE values. |
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