| Cao Hua,Ge Zhongliang,Zhang Qunwei.Cloning and prokaryotic expression of rhTPO in prokaryotic cells[J].Chinese Journal of Radiological Medicine and Protection,1997,17(5):316-318 |
| Cloning and prokaryotic expression of rhTPO in prokaryotic cells |
| Received:December 22, 1995 Revised:April 29, 1996 |
| DOI： |
| KeyWords:Thrombopoietin Prokaryotic expression Cloning |
| FundProject: |
| Author Name | Affiliation | | Cao Hua | Beijing Institute of Radiation Medicine, Beijing 100850, China | | Ge Zhongliang | Beijing Institute of Radiation Medicine, Beijing 100850, China | | Zhang Qunwei | Beijing Institute of Radiation Medicine, Beijing 100850, China | | 孙丽亚 | Beijing Institute of Radiation Medicine, Beijing 100850, China | | 张雪峰 | Beijing Institute of Radiation Medicine, Beijing 100850, China | | 丁清明 | Beijing Institute of Radiation Medicine, Beijing 100850, China |
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| Abstract:: |
| Objectives Thrombopoietin (TPO) is a normal physiological hematopoietic factor which is able to stimulate the hematopoietic progenitor cells to proliferate and differentiate into megakaryocytes and to induce the production and release of platelets. In this study rhTPO was cloned to be potentially used for treatment of radiation-induced thrombocytopenia, we studied the prokaryotic expression of rhTPO.Methods According to the cDNA sequence encoding hTPO published elsewhere, we designed two sets of PCR primers at first, and cloned hTPO gene from fetal liver mRNA by means of RT PCR, then subcloned it into prokaryotic expression vector pBV220 to form recombinant expression plasmids pBV220/TPO, and to transfer it into prokaryotic cells escherichia coli, DH5α. Expression was induced by heat of 42℃. Results We obtained the aimed prokaryotic expression of recombinant(r) hTPO by assessment of SDS-Page. Conclusion hTPO can be expressed completely in prokaryotic cells. The prokaryotic expression of rhTPO might be a basis for the bio treatment of radiation induced thrombocytopenia. |
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