| Wu Wei,Kong Xiangtao,Li Li.Characterization of expression of CD95 of splenic lymphocytes in sublethally γ irradiated mice[J].Chinese Journal of Radiological Medicine and Protection,1997,17(5):305-308 |
| Characterization of expression of CD95 of splenic lymphocytes in sublethally γ irradiated mice |
| Received:December 11, 1996 Revised:April 02, 1997 |
| DOI： |
| KeyWords:Splenic lymphocytes Apoptosis CD95 Whole body irradiation |
| FundProject:全军医学卫生科研基金 |
| Author Name | Affiliation | | Wu Wei | Clinical Immunology Center of PLA, Shanghai Changzheng Hospital, The Second Military Medical University, Shanghai 200433, China | | Kong Xiangtao | Clinical Immunology Center of PLA, Shanghai Changzheng Hospital, The Second Military Medical University, Shanghai 200433, China | | Li Li | Clinical Immunology Center of PLA, Shanghai Changzheng Hospital, The Second Military Medical University, Shanghai 200433, China | | 张玲珍 | Clinical Immunology Center of PLA, Shanghai Changzheng Hospital, The Second Military Medical University, Shanghai 200433, China | | 施笑梅 | Clinical Immunology Center of PLA, Shanghai Changzheng Hospital, The Second Military Medical University, Shanghai 200433, China | | 杨如俊 | 放射医学研究所 | | 闵锐 | 放射医学研究所 |
|
| Hits: 2274 |
| Download times: 1552 |
| Abstract:: |
| Objective The aim of this study was to investigate the apoptosis of splenic lymphocytes and CD95 expression in 60Co γ irradiated mice. Methods Mice were whole body irradiated with 2.0, 4.0, 6.0 Gy γ-rays. The splenic lymphocytes were analyzed by flow cytometry with fluorecscent monoclonal antibodies and PI in different times after irradiation. Results It was found that apoptosis occurred in the normal splenic tissues, Fas was expressed in resting mouse splenic lymphocytes and the expression varied in T cells and B cells. B cells expressed high levels of Fas than T cells. Within 2 hours after 4Gy irradiation, however, B cells and T cells upregulated Fas expression and thereafter downregulated the expression and died by apoptosis if Fas was crosslinked. Conclusions These observations suggest that the radiosensitivity varies with different doses of irradiation in mouse splenic lymphocytes. Fas mediated apoptosis plays a major role after irradiation of aplenic lymphocytes. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
