| Liu Guolian,Zhou Xiaoxia,Zhou Shuzhen.Comparison of malignant transformation of cells vitro induced by high LET and low LET radiations[J].Chinese Journal of Radiological Medicine and Protection,1997,17(3):186-188 |
| Comparison of malignant transformation of cells vitro induced by high LET and low LET radiations |
| Received:July 04, 1996 Revised:December 18, 1996 |
| DOI： |
| KeyWords:Malignant transformation Cells α-particles X rays Lung fibroblasts RBE |
| FundProject: |
| Author Name | Affiliation | | Liu Guolian | Institute of Radiation Medicine, Beijing 100850, China | | Zhou Xiaoxia | Institute of Radiation Medicine, Beijing 100850, China | | Zhou Shuzhen | Institute of Radiation Medicine, Beijing 100850, China | | 樊飞跃 | Institute of Radiation Medicine, Beijing 100850, China | | 杨素霞 | Institute of Radiation Medicine, Beijing 100850, China | | 普琳 | Institute of Radiation Medicine, Beijing 100850, China | | 吴德昌 | Institute of Radiation Medicine, Beijing 100850, China |
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| Abstract:: |
| Objective To compare the characteristics of malignant transoformation of the adult Wistar rat lung fibroblast cell line (WAL F 1) in vitro induced by high LET (238Pu alpha particles, 5 25 MeV) and low LET (X-rays 180 kV) radiations. Methods A model of cell transformation in vitro was used;the changes of morphological and biological characteristics of transformed cell induced by two types of radiations were observed;the carcinogenesis of transformed cells in vivo was studied. Results The results showed that the morphological and biological characteristics of transformed cells were basically similar.However, there were some differences between biological effects of cell transformation induced by these radiation types:(1) The model of cell surviving curve for alpha particles was a single hit and single target model with a D0 value of 0 172 Gy, while that for X-rays was a single hit and multi target model with a D0 value of 1 66 Gy;(2) the iso effect dose of proliferative ability, chromosome aberration rate, and colony forming rate for X-rays were 10 times higher than these for alpha particles;(3) the latent period of cell transformation for alpha particles was delayed about 4 5 passages as compared with that for X-rays;and (4) the relative biological effectiveness (RBE) for alpha particles versus X-rays was approximately 6.0 at an alpha dose of 0.5 Gy. Conclusion The results suggest that the high LET radiation showed higher carcinogenic effect in vitro on cells than low LET radiation did. |
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