| Yang Meiying,Ye Changqing,Wu Dechang,et al.Relative gene in immortalized human fetal tracheal fibroblasts malignantly transformed by alpha particle irradiation[J].Chinese Journal of Radiological Medicine and Protection,1997,17(3):166-168 |
| Relative gene in immortalized human fetal tracheal fibroblasts malignantly transformed by alpha particle irradiation |
| Received:September 02, 1995 Revised:November 19, 1996 |
| DOI： |
| KeyWords:Alpha particles Immortalized human fetal tracheal fibroblasts Relative gene |
| FundProject:国家自然科学基金 |
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| Abstract:: |
| Objectives To explore the relative novel genes which are associated with radiation-induced neoplastic transformation of cells. Mothods The experiment was carried out by reverse transcription of mRNA from SHTF and α SHTF cells, amplification of cDNA by PCR using 24 pairs of primers consisting of 3'[HT11M(G, C, A)] and arbitrary 5' (HAP17～24) primers, tracing with 32P-dATP, running on 6% sequencing gels in adjacent lanes, and then autoradiographing. Results Twenty three DNA segments showed expression in α SHTF cells, but not in SHTF cells. By reamplification of 12 differential expression DNA segments of them at random, 6 DNA segments were obtained.Northern dots showed that C17 5 and C23-1 had positive responses in α SHTF cells, but negative in SHTF cells. Conclusions The research indicates that there is difference in gene expression between SHTF cells and SHTF cells. |
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