| Zhou Pingkun,Sui Jianli and Yang Suhong.Hormetic effect of low dose radiation on cellular DNA repair[J].Chinese Journal of Radiological Medicine and Protection,1997,17(3):155-158 |
| Hormetic effect of low dose radiation on cellular DNA repair |
| Received:December 24, 1996 Revised:January 19, 1997 |
| DOI： |
| KeyWords:Low dose radiation Hormesis DNA repair Gene mutation DNA bound protein |
| FundProject:国际原子能机构协作项目 |
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| Abstract:: |
| Objective To study radio adaptive response of mutagenesis and repair of DNA double-strand breaks (DSB) in mammalian cells induced by low dose γ-rays, and to detect low dose radiation-induced proteins. Methods Mouse SR-1 Cells were irradiated with 60Coγ-rays.Mutations at hprt locus were assayed by 6-thioguanine selective culture method.DNA DSBs were measured by pulsed field gel electrophoresis.Southwestern blot hybridization was employed to detect radiation induced proteins.Results Preexposure of SR-1 cells to 1 cGy at 18h and 24h before challenging dose significantly decreased the frequency of hprt gene mutations induced by following 3 Gy challenge.Preexposure of SR-1 cells to single 1 cGy as well as to 1 cGy per day for 10 successive days significantly increased the cellular capacity of rejoining 3 Gy induced DNA DSBs.A newly synthesized protein bound to damaged DNA was detected at 16h after 1 cGy exposure. Conclusion Proteins possibly involved in the process of DNA repair were induced by low dose radiation upregulating the expression of some specific genes.Such induced proteins lead to increase of cellular DNA repair capacity as well as radio adaptive response of cells to gene mutations. |
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