| Cao Hua,Ge Zhongliang,Liu Xiuzhen.Influence of rhTPO on hematopoiesis in mice irradiated with 60Co γ-ray[J].Chinese Journal of Radiological Medicine and Protection,1997,17(3):151-154 |
| Influence of rhTPO on hematopoiesis in mice irradiated with 60Co γ-ray |
| Received:November 28, 1996 Revised:December 05, 1996 |
| DOI： |
| KeyWords:Thrombopoietin Megakaryocytopoiesis Biological activity |
| FundProject: |
| Author Name | Affiliation | | Cao Hua | Institute of Radiation Medicine, Beijing 100850, China | | Ge Zhongliang | Institute of Radiation Medicine, Beijing 100850, China | | Liu Xiuzhen | Institute of Radiation Medicine, Beijing 100850, China | | 胡良平 | 北京情报研究所 | | 张群伟 | Institute of Radiation Medicine, Beijing 100850, China | | 孙丽亚 | Institute of Radiation Medicine, Beijing 100850, China | | 张雪峰 | Institute of Radiation Medicine, Beijing 100850, China |
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| Abstract:: |
| Objective Thromboietin, a major physiological regulator of megakaryocytopoiesis, has potent effects on megakaryocytopoiesis. With a view to finding a new therapeutic modality for bone marrow injury induced thrombocytopenia, we studied the biological activity of rhTPO in vivo. Methods We recombined hTPO by means of RT-PCR and eukaryotic expression. The rhPTO was administered to 5.0Gy 60Co γ-ray irradiated male mice, once per day for 20 consecutive days. Peripheral blood counts were monitored and bone marrow was assayed for clonogenic activity. Results Circulating platelet counts and CFU-MK of the rhTPO-treated mice increased significantly in contrast with the control group. Conclusion These results show that rhTPO can restore platelets and CFU-MK of irradiated mice in vivo. Clearly, our growing understanding of the biology of TPO will lead to important new insights into megakaryopoiesis and to improvement in clinical treatment of bone marrow failure. |
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