| Pei Xuetao,Wang Lisheng,Xu Li.Qualitative and quantitative studies of human umbilial cord blood hematopoietic stem cells[J].Chinese Journal of Radiological Medicine and Protection,1997,17(3):146-150 |
| Qualitative and quantitative studies of human umbilial cord blood hematopoietic stem cells |
| Received:November 25, 1996 Revised:December 11, 1996 |
| DOI： |
| KeyWords:Human umbilial cord blood Hematopoietic stem cells Proliferation and differentiation Expansion Grafting efficiency |
| FundProject:国家863计划项目基金 |
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| Abstract:: |
| Objective To evaluate the quality and quantity of human umbilial cord blood hematopoietic stem cells for transplantation, the percentage, abilities of proliferation and differentiation, expansion responses and grafting efficiency of CD34+ cells and their subpopulations from umbilical cord blood were studied.Methods CD34+ cells and their subsets (CD34+CD38+ and CD34+CD38-) were isolated from human umbilical cord blood by using MACS or FACS system, cultured and expanded with hematopoietic growth factors (HGFs) in semi solid and liquid culture systems, and the grafting efficiency was evaluated by Panning assay. Results The percentage of CD34+CD38- subset in cord blood was much higher (9 5%) than that in bone marrow or peripheral blood (4%) (P<0.01 ). More hematopoietic colony formation from cord blood than that from bone marrow or peripheral blood was observed. With addition of HGFs, the total uncleated cells increased by 500.0±82.5-fold, the hematopoietic progenitor cells by 94.1±28.6-fold and the CD34+ cells by 24.0±3.8-fold. Only 36% of CD34+ cells were taken in a pre formed adult bone marrow stroma in a short time (2hr), but 68%-89.6% of those pretreated with GM CSF and / or IL 3 for 6-8hr were taken in the stroma (P<0.01 ). Conclusions It is possible to expand cord blood CD34+ cells ex vivo to improve their grafting efficiency for utilization of cord blood stem cells in treatment of acute radiation sickness, transplantation and therapeutic gene transfer. |
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