| GONG Yifen,GUO Renfeng,ZHU Maoxiang,SHOU Jiang,L. Hieber,K. Peters,C. Schippel,GE Guixiu,YANG Zhihua.Enhancement of alpha particles induced cell transformation by oxygen free radicals and tumor necrosis factor released from phagocytes[J].Chinese Journal of Radiological Medicine and Protection,1997,17(2):98-101 |
| Enhancement of alpha particles induced cell transformation by oxygen free radicals and tumor necrosis factor released from phagocytes |
| Received:October 10, 1995 Revised:May 06, 1996 |
| DOI： |
| KeyWords:Cell Transformation Alpha particles Oxygen free radicals Tumor necrosis factor Phagocytes |
| FundProject:本课题获国家自然科学基金（No39170273）资助 |
| Author Name | Affiliation | | GONG Yifen | Institute of Radiation Medicine, Beijing 100850, China | | GUO Renfeng | Institute of Radiation Medicine, Beijing 100850, China | | ZHU Maoxiang | Institute of Radiation Medicine, Beijing 100850, China | | SHOU Jiang | Institute of Radiation Medicine, Beijing 100850, China | | L. Hieber | GSF Institute of Radiology, Munich, Germany | | K. Peters | GSF Institute of Radiology, Munich, Germany | | C. Schippel | GSF Institute of Radiology, Munich, Germany | | GE Guixiu | Institute of Radiation Medicine, Beijing 100850, China | | YANG Zhihua | Institute of Radiation Medicine, Beijing 100850, China |
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| Abstract:: |
| Objective To illustrate the role of several endogenous factors released from phagocytes under chronic inflammation in radiation-induced cancer.Methods C3T10T1/2 and SHE cells were used as targets,and 238Pu alpha source was used in alpha irradiation .The enhancement of TF in alpha particles induced cell transformation by PMA stimulated human blood and zymosan stimulated U 937 cells was studied using formation of transformed foci.Results Transformation frequency （TF） of C3H10T1/2 cells exposed to alpha particles of 0.5 Gy increased 2.1 and 2.8 fold by PMA and PMA stimulated neutrophils,respectively.TF of irradiated SHE cells at a dose of 0.5 Gy increased 12 fold by the addition of the supernatant of macrophage like U 937 cell line.It was shown that TF of irradiated SHE cells at above dose increased 8 fold by the supernatant treated with anti TNF α antibody and there were unknown promoters in it.The irradiated SHE cells treated with human recombinant TNF α could be subcultured continuously in vitro.The cells at 40th passage and two lines of monoclone cells have the ability to develop malignant tumors in nude mice.Conclusion The overdose of free radicals and TNF α released from neutrophils and macrophages have played an important role in low dose radiation-induced cancer. |
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