| Min Rui,Liu Benti,Cheog Tianmin,Yang Rujun,Meng Xiangshun.Blot hybridization analysis of TCR genes of T cells for five people exposed in a radiation accident[J].Chinese Journal of Radiological Medicine and Protection,1996,16(6):405-408 |
| Blot hybridization analysis of TCR genes of T cells for five people exposed in a radiation accident |
| Received:June 06, 1995 Revised:February 28, 1996 |
| DOI： |
| KeyWords:TCR gene ionizing radiation blot hybridization |
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| Abstract:: |
| Human lymphocyte total DNA was prepared in agarose plug by mixing cells with low melting agarose, and two restriction endonucleases were used for digestion of the total DNA with human a and β TCR cDNA probes. The total digested DNA from five people who were whole body exposed to 2.0-2.5 Gy ionizing radiation in an accident 4.5 years ago was hybridized by Southern blot method. The results showed that no obvious difference in hybridization bands was found between controls and the five victims when hybridizations were fulfilled in the total DNA which was digested by Hind Ⅲ restriction endonuclease with both a and β probes. However, when the total DNA was digested with restriction endonuclease EcoRⅠ and was hybridized with TCR a probe, four of the five exposed people showed a different hybridizing band pattern comparied with the controls. The results are also discussed. |
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