| Sun Kai.Effects of interleukin-11 gene therapy on hematopoiesis of irradiated mice[J].Chinese Journal of Radiological Medicine and Protection,1996,16(5):310-313 |
| Effects of interleukin-11 gene therapy on hematopoiesis of irradiated mice |
| Received:November 19, 1995 Revised:February 26, 1996 |
| DOI： |
| KeyWords:Interleukin-11 Gene therapy Hematopoiesis |
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| Abstract:: |
| interleukin-11 is a multifunctional hematopoietic growth factor, especially in hematopoiesis.It can act synergistically with IL-3 to stimulate hematopoiesis at multiple stages by shortening the G0 period of hematopoietic progenitor cells, and has a very strong effect on increasing perpheral platelet level.This fact suggests that IL-11 might be a potential hematopoiesis-promoting drug.In order to identify the availability of IL-11 gene therapy in hematopoiesis disorders, a retroviral vector carrying IL-11 cDNA was constructed and transfected into murine fibroblast cell line NIH-3T3.After G418 selection and cloning, we obtained a subclone 1E7 secreting high level of IL-11, and we transplanted 1E7 cells intraperitoneally into BALB/c mice to inverstigate its effects on peripheral blood cell count and bone marrow colony formation of sublethally irradiated mice.Results showed that IL-11 gene therapy had significant effects on both normal and sublethally irradiated mice including an increase in the peripheral platelets of normal mice and marked acceleration of the recovery of peripheral WBC and platelets in sublethally irradiated mice.This results suggested that IL-11 might play an important role in the regulation of hematopoiesis and IL-11 gene therapy had a potential value in hematopoiersis recovery. |
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