Wei Handong.Effects of Bcl-2 gene transfer on proliferation and irradiation sensitivity of NIH3T3 cells[J].Chinese Journal of Radiological Medicine and Protection,1996,16(5):290-292
Effects of Bcl-2 gene transfer on proliferation and irradiation sensitivity of NIH3T3 cells
Received:April 17, 1996  Revised:June 28, 1996
DOI：
KeyWords:Retrovirus  Gene transfer  Bcl-2  gene Irradiation  sensitivity  Cell proliferation
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Author NameAffiliation
Wei Handong Institute of Radiation Medicine, Beijing, 100850 
瞿成奎 Institute of Radiation Medicine, Beijing, 100850 
贺福初 Institute of Radiation Medicine, Beijing, 100850 
吴祖泽 Institute of Radiation Medicine, Beijing, 100850 
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Abstract::
      The packaging cell line PA317 was transfected with the recombinant retrovirus vector carrying Bcl-2.After 15 days, G-418-resistant clones were isolated and expanded, and the culture supernatant was collected to infect NIH 3 T 3.Compared with the control group, the infected NIH 3 T 3 cells were higher in agarose colony forming ability and proliferation and lower in irradiation sensitivity.
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