| Shi Jianhui,Cheng Wenying,Yang Xing,et al.Evaluation of radiosensitivity of nasopharyngeal cancer cells by in situ nick translation[J].Chinese Journal of Radiological Medicine and Protection,1996,16(3):173-175 |
| Evaluation of radiosensitivity of nasopharyngeal cancer cells by in situ nick translation |
| Received:March 21, 1995 Revised:July 14, 1995 |
| DOI： |
| KeyWords:In situ nick translation nasopharyngeal carcinoma cell line radiosensitivity |
| FundProject: |
| Author Name | Affiliation | | Shi Jianhui | Shanghai Institute of Radiation Medicine, Shanghai Medical Umiversity, Shanghai, 200032 | | Cheng Wenying | Shanghai Institute of Radiation Medicine, Shanghai Medical Umiversity, Shanghai, 200032 | | Yang Xing | Shanghai Institute of Radiation Medicine, Shanghai Medical Umiversity, Shanghai, 200032 | | 罗伟华 | Shanghai Institute of Radiation Medicine, Shanghai Medical Umiversity, Shanghai, 200032 | | 王建平 | 200032 上海, 上海医科大学放射医学研究所 | | 徐萍 | Shanghai Institute of Radiation Medicine, Shanghai Medical Umiversity, Shanghai, 200032 |
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| Abstract:: |
| We evaluated the radiosensitivity of two nasopharyngeal cell lines which had been treated with γ-rays by in situ nick translation(ISNT).The results indicated that the incorporation rate of dNTP of thetwo cell lines increased with the radiation dose.Good correlations were found between the radiosensitivity determined by the in situ nick translation assay andconfirmed by the colony-forming assay in CNE and CNE-2Z cell lines.Although this report deals with cultured cells, the described technique of datecting DNA nickin situ without destruction of morphology seems to be applicable to tissue section.It may be possible to use it as a tool for predicting radiosensitivity of cancerous tissues treated with γ-rays. |
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