| Cai Jianming,Zheng Xiolong,Luo Chengji.Effect of γ-rays on rodent DNA polymerase βactivity[J].Chinese Journal of Radiological Medicine and Protection,1995,15(5):326-329 |
| Effect of γ-rays on rodent DNA polymerase βactivity |
| Received:May 10, 1994 Revised:October 06, 1994 |
| DOI： |
| KeyWords:DNA polymerase β activity radiation effects gamma rays rodents |
| FundProject:国家自然科学基金 |
| Author Name | Affiliation | | Cai Jianming | Department of Radiation Medicine, Second Military Medical University, Shanghai, 200433 | | Zheng Xiolong | Department of Radiation Medicine, Second Military Medical University, Shanghai, 200433 | | Luo Chengji | 630083 重庆, 第三军医大学防原医学教研室 | | 高建国 | Department of Radiation Medicine, Second Military Medical University, Shanghai, 200433 | | 程天民 | 630083 重庆, 第三军医大学防原医学教研室 | | 余宏宇 | Department of Radiation Medicine, Second Military Medical University, Shanghai, 200433 | | 杨如俊 | Department of Radiation Medicine, Second Military Medical University, Shanghai, 200433 | | 张丽民 | Department of Radiation Medicine, Second Military Medical University, Shanghai, 200433 |
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| Abstract:: |
| The effect of γ-rays on rodent DNA polymerase βactivity was studied with 3H-TTP incorporation inhibition test, using Nethylmaleimide as the selective inhibitor of DNA polymerase αand γ.The DNA polymerase βtested here included recombinant rat enzyme,enzyme in nuclei of normal hepatocytes and SMMC-LTNM hepatoma from BALB/c nude mice.It was found that the rat recombinant polymerase βactivity decreased markedly as the absorbed dose increased to over 40Gy.The enzyme activity in both kinds of nuclei was not significantly affected after exposure to γ-rays in vitro, whereas it decreased slightly within 24-48 hours after total body irradiation with 2 Gy of γ-rays, and then temporarily increased to over normal level. |
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