| Cai Jianming,ZhengXiulong,Gao Jianguo,et al.Assay of DNA polymerase β-activity and its application[J].Chinese Journal of Radiological Medicine and Protection,1995,15(2):99-102 |
| Assay of DNA polymerase β-activity and its application |
| Received:April 05, 1994 Revised:July 06, 1994 |
| DOI： |
| KeyWords:DNA polymerase β-activity enzyme test radiation effect nude mice enzyme inhibitors |
| FundProject:国家自然科学基金 |
| Author Name | Affiliation | | Cai Jianming | Department of Radiation Medicine, SecondMititary Medical University, Shanghai 200433 | | ZhengXiulong | Department of Radiation Medicine, SecondMititary Medical University, Shanghai 200433 | | Gao Jianguo | Department of Radiation Medicine, SecondMititary Medical University, Shanghai 200433 | | 罗成基 | 重庆, 第三军医大学防原教研室 | | 程天民 | 重庆, 第三军医大学防原教研室 | | 余宏宇 | 重庆, 第三军医大学防原教研室 | | 杨如俊 | Department of Radiation Medicine, SecondMititary Medical University, Shanghai 200433 |
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| Abstract:: |
| A simple method of measuring DNA polymerase β-activity was established. This method is based on the following principle: DNA polymerases can enhance the ability of incorporating 3H-TTP into double-strand DNA gaps of activated DNA.In the condition of using selective inhibitor N-ethylmaleimide,which inhibits other DNA polymerases without effecting DNA polymerase β-activity,the incorporation rate of 3H-TTP in activated DNA can be considered as the reflection of DNA polymerase β-activity.Using this method,it was found that the β-activity of DNA polymerase in SMMC-LTNM hepatoma cells was two-fold higher than that in normal hepatocytes of nude BALB/cmice,and that the β-activity of DNA polymerase was not significantly affected by γ-rays within 30 Gy.This finding implies that the insensitivity to irradiation of some tumour cells might be related to their high DNA polymerase β-activity. |
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