| 黎晏卿,江其生,史更生,田思琦,张蓉,程菲,崔凤梅,涂彧,陈慧芹.中药组合物对氡暴露小鼠肺组织及支气管上皮细胞损伤的防护作用[J].中华放射医学与防护杂志,2021,41(8):587-594 |
| 中药组合物对氡暴露小鼠肺组织及支气管上皮细胞损伤的防护作用 |
| Protective effect of the Chinese medicine composition on the radon exposure induced injuries in mice lung and bronchial epithelial cells |
| 投稿时间：2021-04-08 |
| DOI：10.3760/cma.j.issn.0254-5098.2021.08.005 |
| 中文关键词: 氡暴露 中药组合物 支气管上皮细胞 辐射防护 |
| 英文关键词:Radon exposure Chinese medicinal composition Bronchial epithelial cells Radiation protection |
| 基金项目:军队后勤研究项目（AEP17J001）；江苏省大学生创新创业训练项目（201910285045Z） |
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| 中文摘要: |
| 目的 在体内外实验中探讨中药组合物夏丹芪（冬虫夏草、丹参、黄芪）对小鼠氡暴露肺组织损伤的防治效果。方法 建立氡暴露小鼠模型，分为健康对照组、氡暴露组和夏丹芪干预氡暴露组，每组6只。HE和Masson染色观察120 WLM时各组小鼠肺组织病理变化，免疫组织化学法检测肺组织α-SMA蛋白和Vimentin蛋白表达；超氧化物歧化酶（SOD）和丙二醛（MDA）试剂盒检测各组肺组织氧化应激水平。同时，利用生态氡室构建氡暴露细胞模型和氡暴露+夏丹芪干预细胞模型，粘附试剂盒检测不同组细胞粘附能力；Transwell迁移实验确定各组细胞迁移能力；Western blot法检测各组细胞E-cadherin和Vimentin蛋白的表达情况。结果 与氡暴露组相比，2 mg/g夏丹芪干预可使氡暴露小鼠肺组织MDA浓度降低（t=4.43，P<0.05），SOD活性升高（t=3.22，P<0.05）；肺组织α-SMA和Vimentin蛋白表达降低（t=3.08、7.57，P<0.05）。在体外实验中，与单纯氡暴露组相比，150和200 μg/ml夏丹芪干预后氡暴露细胞迁移能力下降（t=4.78、13.01，P<0.05），细胞粘附性能增强（t=3.41、12.55，P<0.05）；E-cadherin蛋白表达增高（t=2.96、19.57，P<0.05），Vimentin蛋白表达明显下降（t=21.00、33.32，P<0.05）。结论 中药组合物夏丹芪可通过降低辐射诱发的氧化应激、减弱氡暴露诱发的上皮细胞间质转化和纤维化，对氡暴露损伤具有一定的辐射防护作用，有望成为氡损伤的辐射防护剂。 |
| 英文摘要: |
| Objective To explore the effect of a Chinese medicinal composition (Xiadanqi) on the prevention of radon exposure induced injuries of lung in vitro and in vivo.Methods Mice were randomly divided into three groups of blank control group, radon-exposed group alone and radon-exposed group intervened with Chinese medicinal composition. The pathological changes of lung tissues in each group after 120 WLM were observed by HE and Masson staining, and the expressions of α-SMA protein and Vimentin protein in lung tissues were detected by immunohistochemistry staining. The levels of oxidative stress in lung tissue of each group were detected with SOD and MDA kits. At the same time, a radon exposed cell model and a radon exposure + Xiadanqi intervention cell model were constructed using an ecological radon chamber. The cell adhesion abilities of different groups were detected by an adhesion kit. The cell migration ability of each group was determined by the transwell migration experiment. The expression of E-cadherin and Vimentin protein was detected by Western blot.Results Compared with the radon exposure group, the concentration of MDA was decreased (t=4.43, P<0.05), the activity of SOD was increased (t=3.22, P<0.05), and α-SMA and Vimentin protein expressions were decreased (t=3.08, 7.57, P<0.05) in lung tissue of mice intervened with 2 mg/g Xiadanqi. In vitro, compared with radon exposure group, the migration ability was reduced (t=4.78, 13.01, P<0.05), the cell adhesion property was enhanced (t=3.41, 12.55, P<0.05), the expression of E-cadherin protein was increased (t=2.96, 19.57, P<0.05), and the expression of Vimentin protein was obviously reduced (t=21.00, 33.32, P<0.05) in radon-exposed cells with the treatment of Chinese medicine (150 μg/ml and 200 μg/ml).Conclusions The Chinese medicinal composition (Xiadanqi) has a certain radioprotective effect on radon exposure induced injury by reducing oxidative stress, attenuating EMT and fibrosis, and thus it may be applied as a protective agent for radon induced injury. |
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