| 路璐,张俊伶,李德冠,樊赛军.禁食对137Cs γ射线照射诱导小鼠肠道辐射损伤的代谢组学研究[J].中华放射医学与防护杂志,2021,41(8):564-570 |
| 禁食对137Cs γ射线照射诱导小鼠肠道辐射损伤的代谢组学研究 |
| Metabonomic study on the effect of fasting on 137Cs γ-ray radiation-induced intestinal injury in mice |
| 投稿时间：2020-11-11 |
| DOI：10.3760/cma.j.issn.0254-5098.2021.08.002 |
| 中文关键词: 禁食 辐射损伤 代谢组学 |
| 英文关键词:Fasting Radiation injury Metabolomics |
| 基金项目:国家自然科学基金面上项目（81703169，81572969，81730086）；天津市科技支撑计划项目（14ZCZDSY00001） |
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| 中文摘要: |
| 目的 研究禁食对137Cs γ射线照射诱导小鼠肠道辐射损伤的干预作用，通过非靶向代谢组学探究小鼠粪便代谢物的变化。方法 将小鼠分为健康对照组、γ射线照射（全身9 Gy或腹部15 Gy）组、禁食（24、48、72 h）+照射（全身9 Gy或腹部15 Gy）组。照射后，计算小鼠的生存率、脾脏指数和胸腺指数。非靶代谢实验测序分为4组，分别为健康对照组、禁食24 h组、腹部局部照射15 Gy组、禁食24 h组+腹部局部照射15 Gy组、每组6只。于照后3.5 d收集各组小鼠的粪便进行非靶向代谢组学检测。结果 9 Gy γ射线全身照射小鼠照射前禁食48和24 h，受照后的中位生存期提高了1和4 d；15 Gy腹部受照小鼠照射前禁食48和24 h的小鼠的存活率分别为16.67%和25%，照射前禁食24 h能够提高受照后3.5 d小鼠的体重（t=2.338，P=0.042）和脾脏指数（t=2.289，P=0.045）。非靶向代谢组学结果显示，禁食24 h和未禁食的受腹部局部照射小鼠粪便样本中有30个差异表达代谢物；代谢通路富集分析表明，类固醇激素生物合成的代谢途径存在着不平衡状态。结论 照射前禁食可以提高肠道辐射损伤小鼠的生存率，改变其肠道代谢产物，提示照射前禁食或短期内饮食营养变化参与调节肠道辐射损。 |
| 英文摘要: |
| Objective To study the effect of fasting on 137Cs γ-ray radiation-induced intestinal injury in mice, and to explore the effect of fasting on fecal metabolites of mice through non-targeted metabolomics.Methods C57BL/6 mice were divided into healthy control group, 9 Gy γ-ray whole body irradiation (WBI)/15 Gy γ-ray whole abdominal irradiation (WAI) group, fasting (24 h, 48 h, 72 h)+ 9 Gy WBI/15 Gy WAI group. After irradiation, the survival rate, spleen index and thymus index were calculated. C57BL/6 mice in non-target metabolism experiment were randomly divided into four groups:control group, fasting 24 h group, 15 Gy γ-ray WAI group, fasting 24 h + 15 Gy γ-ray WAI group, 6 mice in each group. After 15 Gy WAI, the feces of mice in each group were collected at 3.5 days for non-targeted metabolomics detection.Results The median survival time of mice with 48 h and 24 h fasting before 9 Gy γ-ray irradiation was increased by 1 day and 4 days, and the survival rates of mice treated with 48 h and 24 h fasting before 15 Gy WAI were 16.67% and 25%, respectively. 15 Gy γ-ray WAI on mice with fasting for 24 h before irradiation could increase the body weight (t=2.338, P=0.042) and spleen index (t=2.289, P=0.045) at 3.5 days after irradiation. Through non-targeted metabonomic analysis, it was found that there were 30 differentially expressed metabolites in fecal samples of fasting and non-fasting mice subjected to WAI, and metabolic pathway enrichment analysis showed that there was an imbalance in the metabolic pathway of steroid biosynthesis.Conclusions Fasting before irradiation can improve the survival rate of mice with intestinal radiation injury and change their intestinal metabolites, suggesting that pre-irradiation fasting or short-term dietary nutrition changes are involved in the regulation of intestinal radiation damage. |
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